Our lab icon Our Lab

13th floor garden stairs

Our lab is located in The Donnelly Centre for Cellular and Biomolecular Research at the University of Toronto. We have access to several state-of-the-art facilities including robotic facilities, high content screening microscopes and sequencing facilities.

Publications icon Publications

Global Genetic Networks and the Genotype-to-Phenotype Relationship.
Costanzo M, Kuzmin E, van Leeuwen J, Mair B, Moffat J, Boone C, Andrews B. 2019, Cell, 21;177(1):85-100.
Exploring genetic suppression interactions on a global scale.
van Leeuwen J, Pons C, Mellor JC, Yamaguchi TN, Friesen H, Koschwanez J, Ušaj MM, Pechlaner M, Takar M, Ušaj M, VanderSluis B, Andrusiak K, Bansal P, Baryshnikova A, Boone CE, Cao J, Cote A, Gebbia M, Horecka G, Horecka I, Kuzmin E, Legro N, Liang W, van Lieshout N, McNee M, San Luis BJ, Shaeri F, Shuteriqi E, Sun S, Yang L, Youn JY, Yuen M, Costanzo M, Gingras AC, Aloy P, Oostenbrink C, Murray A, Graham TR, Myers CL, Andrews BJ, Roth FP, Boone C. 2016, Science, 354(6312).
Exploring Quantitative Yeast Phenomics with Single-Cell Analysis of DNA Damage Foci.
Styles EB, Founk KJ, Zamparo LA, Sing TL, Altintas D, Ribeyre C, Ribaud V, Rougemont J, Mayhew D, Costanzo M, Usaj M, Verster AJ, Koch EN, Novarina D, Graf M, Luke B, Muzi-Falconi M, Myers CL, Mitra RD, Shore D, Brown GW, Zhang Z, Boone C, Andrews BJ. 2016, Cell Syst., 3(3):264-277.e10.
A global genetic interaction network maps a wiring diagram of cellular function.
Costanzo M, VanderSluis B, Koch EN, Baryshnikova A, Pons C, Tan G, Wang W, Usaj M, Hanchard J, Lee SD, Pelechano V, Styles EB, Billmann M, van Leeuwen J, van Dyk N, Lin ZY, Kuzmin E, Nelson J, Piotrowski JS, Srikumar T, Bahr S, Chen Y, Deshpande R, Kurat CF, Li SC, Li Z, Usaj MM, Okada H, Pascoe N, San Luis BJ, Sharifpoor S, Shuteriqi E, Simpkins SW, Snider J, Suresh HG, Tan Y, Zhu H, Malod-Dognin N, Janjic V, Przulj N, Troyanskaya OG, Stagljar I, Xia T, Ohya Y, Gingras AC, Raught B, Boutros M, Steinmetz LM, Moore CL, Rosebrock AP, Caudy AA, Myers CL, Andrews B, Boone C. 2016, Science, 353(6306).
Yeast Proteome Dynamics from Single Cell Imaging and Automated Analysis.
Chong YT, Koh J L-Y, Friesen H, Kaluarachchi S, Cox MJ, Moses AM, Moffat J, Boone C, and Andrews BJ. 2015, Cell, 161(6):1413-24.

Areas of research icon Research

Our lab uses synthetic genetic array (SGA) technology combined with a variety of libraries of yeast strains to genetically screen for interactions of enzymes (e.g. protein kinases, lysine deacetylases) and their targets. We create a variety of resources, databases and collections for the community with application to the SGA platform, including a selection of overexpression arrays.
We have combined SGA with high content screening (HCS) to assess changes in protein localization and abundance under a variety of genetic, environmental and chemical stresses using rapid image acquisition and analysis. SGA-HCS is also used to detect mutants that show defects in their sub-cellular morphology, such as reduced formation of DNA damage foci.
Cell cycle regulated transcription
Our lab has pioneered the use of SGA for genome wide analysis of reporter gene-expression, particularly for studying transcriptional changes throughout the cell cycle. We are also interested in understanding cell cycle processes and molecular players that regulate endocytosis via directed mechanistic studies.