Our lab is located in The Donnelly Centre for Cellular and Biomolecular Research at the University of Toronto. We have access to several state-of-the-art facilities including robotic facilities, high content screening microscopes and sequencing facilities.
- Yeast Proteome Dynamics from Single Cell Imaging and Automated Analysis.
- Chong YT, Koh J L-Y, Friesen H, Kaluarachchi S, Cox MJ, Moses AM, Moffat J, Boone C, and Andrews BJ. 2015, Cell, 161(6):1413-24.
- A cell cycle-regulated oscillator coordinates core histone gene transcription by histone acetylation.
- Kurat CF, Lambert J-P, Petschnigg J, Friesen H, Pawson T, Rosebrock A, Gingras A-C, Fillingham J and Andrews BJ. 2014, PNAS 111(39):14124-9.
- Heritability and genetic basis of protein level variation in an outbred population.
- Parts L, Liu Y-C, Tekkedil MM, Steinmetz LM, Caudy AA, Fraser AG, Boone C*, Andrews BJ*, Rosebrock AP*. 2014, Genome Research, 24(8):1363-70. * Co-corresponding authors
- Functional analysis with a barcoder yeast gene overexpression system.
- Douglas AC, Smith AM, Sharifpoor S, Yan Z, Durbic T, Heisler LE, Lee AY, Ryan O, Göttert H, Surendra A, van Dyk D, Giaever G, Boone C, Nislow C, Andrews BJ. 2012, G3, Oct;2(10):1279-89.
- Our lab uses synthetic genetic array (SGA) technology combined with a variety of libraries of yeast strains to genetically screen for interactions of enzymes (e.g. protein kinases, lysine deacetylases) and their targets. We create a variety of resources, databases and collections for the community with application to the SGA platform, including a selection of overexpression arrays.
- We have combined SGA with high content screening (HCS) to assess changes in protein localization and abundance under a variety of genetic, environmental and chemical stresses using rapid image acquisition and analysis. SGA-HCS is also used to detect mutants that show defects in their sub-cellular morphology, such as reduced formation of DNA damage foci.
- Cell cycle regulated transcription
- Our lab has pioneered the use of SGA for genome wide analysis of reporter gene-expression, particularly for studying transcriptional changes throughout the cell cycle. We are also interested in understanding cell cycle processes and molecular players that regulate endocytosis via directed mechanistic studies.