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T*
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0 -1.2203 TD
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T*
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T*
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[(NR)-421.5(progenitor)-424.7(cells)-424.9(were)-425.8(extracted)-422.7(from)-425.1(the)-421.1(developing)]TJ
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[(eye)-396.2(tissues)-388.6(into)-395.9(Dispase)-387.7(\(Collaborative)-395.1(Research,)-396.8(VWR\))-385.9(for)]TJ
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[(1)-170.2(min)-516.6(\(postnatal)-518.6(period\))-517.6(to)-515.4(3)-170.2(min)-510.3(\(adult\))-521(at)-518.5(37)]TJ
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T*
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T*
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T*
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T*
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T*
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T*
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T*
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[(C.)-439.4(Upon)-445.2(enzyme)-441(exposure)-452.1(spheres)]TJ
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0 -1.2266 TD
[(Partially)-244.2(dissociated)-245.3(colonies)-244.1(were)-242.4(then)-241.1(resuspended)-249.1(in)-240.9(SFM-)]TJ
0 -1.2203 TD
[(containing)-422(1)-163.9(mg/mL)-416.5(Trypsin)-416.1(inhibitor)-417.8(and)-416.9(triturated)-414.8(addi-)]TJ
T*
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0 -1.2266 TD
[(again)-364.4(for)-358(5)-170.2(min)-358.6(at)-360.4(1,500)-165.4(rpm)-366.3(and)-360(resuspended)-362.9(in)-361(SFM)]TJ
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[(PND2)-267.8(NR-derived)-268.4(progenitors,)-264.5(PND2)-267.8(pCE-)-264.2(and)-265.1(adult)-260.8(CE-)]TJ
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[(derived)-289(RSC)-287.4(cultures)-286.1(were)-280.3(cultured)-287.8(in)-285.1(SFM)]TJ
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[(of)-357.3(the)-351.5(DMSO)-355.8(vehicle)-351.7(\(Ctl\))-352.9(or)-357.6(in)-348.4(increasing)-360.4(doses)-349.7(of)]TJ
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[(nyl]-S-phenylglycine)-564.2(t-butyl)-552.6(ester;)-549(Calbiochem\))-552(from)-551.6(1)-549.6(t)0(o)]TJ
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[(receptor)-473(inhibitor)-468.3(SU)-467.8(5402)-465.9(\(Calbiochem\))-470.3(from)-463.1(1)-467.4(t)0(o)-464.8(1)0(0)]TJ
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0 -1.2203 TD
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T*
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T*
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[(drug)-521.6(and)-511.7(dose)-519.1(groups,)-520.7(washing)-516.7(them)-519.8(twice)-517(in)-512.8(SFM)-520.2(and)]TJ
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[(in)-310.4(sphere)-303.7(numbers)-305.8(compared)-307.6(to)-306.7(vehicle)-307.5(controls)-305.1(after)-310.9(a)-303(total)]TJ
T*
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[(Knock)-7(down)-340.4(siRN)-8.4(A)-335.5(experi)-7.4(ments)]TJ
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(Targeted)Tj
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(Hes5)Tj
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[(,)-357(and)-347.3(non-targeted)-359.8(control)-354.4(\(scrambled\))-357.9(siR-)]TJ
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[(NA)-418.7(was)-419.8(used)-422.9(at)-417.3(50)-163.4(nM)-419.5(with)-420(DharmaFECT)-417.3(transfection)-426.2(re-)]TJ
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[(agent)-836(1)-827.8(\(Dharmacon\).)-839.7(Knockdown)-832.8(was)-830.8(conrmed)-831.6(by)]TJ
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[(quantitative)-289.2(polymerase)-289.2(chain)-281(reaction)-287.8(\(q-PCR\))-283.5(for)]TJ
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(Hes1)Tj
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(Hes5)Tj
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[(siRNA.)-525.5(No)-514.9(signicant)-527.7(difference)-522.7(in)]TJ
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(Hes1)Tj
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(2)Tj
39.5877 0 TD
[(BALENCI)-334.5(AND)-337.2(VAN)-332.3(DER)-338.6(KOOY)]TJ
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[(Ret)-8.2(rovirus)-340.9(preparat)-8.9(ion)-333.9(and)-335.7(infe)-7.4(ction)]TJ
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[(Replication-incompetent)-400.7(retroviral)-385.6(backbones)-386.2(pMX-IRES-)]TJ
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[(GFP)-617.8(\(pMXIE\))-617.4(and)-612.9(N1ICD-containing)-621.7(retroviral)-613.2(construct)]TJ
T*
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T*
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[(culture)-468.2(media)-465.1(for)-465.5(7)-461.1(days.)-460.1(Numbers)-466.8(of)-464.8(clonal)-467.3(non-infected)]TJ
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[()55(GFP)]TJ
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(-)Tj
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[()55()-330.7(and)-334.7(infected)-338.7()55(GFP)]TJ
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[()55()-330.7(spheres)-336.7(were)-337.2(determined.)]TJ
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[(RTP)-10.7(CR)-333.7(and)-341.4(q-PCR)]TJ
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[(mi)17.9(cr)19(od)19.5(is)20.4(se)17.8(ct)19.2(ed)-306.4(a)13.1(n)0(d)-309.9(R)14.7(NA)-304.9(wa)17.8(s)-317.5(e)12.7(xt)16.3(ra)18.8(c)13.4(t)0(e)18.6(d)-312.8(us)16.3(in)18.4(g)-314.2(R)0(N)21.1(A)0(e)17.7(a)0(s)18.1(y)-314.2(mi)24.2(ni)]TJ
T*
[(ki)17.3(t)-575.9(\()12.2(Qi)18.7(a)13.1(g)0(e)14.7(n)0(\))-560.3(c)0(o)16.9(m)0(p)20(l)0(e)15.5(m)15.1(en)15.7(t)12.2(e)0(d)-565.6(w)0(i)20.1(t)0(h)-566.6(D)0(N)17.6(a)13.1(se)-563.9(tr)17.8(e)12.8(a)0(t)19(m)0(e)15.2(n)0(t)21.4(.)-572(RN)14.8(As)]TJ
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[(we)17.4(re)-481.1(t)12.2(h)0(e)15.7(n)-483.9(re)18.4(ve)18.3(rs)17(e)12.8(-)0(t)18(r)0(a)18.8(n)0(s)20.6(c)0(r)19(i)0(b)17.3(e)0(d)-477.1(u)0(s)16.3(i)0(n)18.4(g)-485(on)19.1(e-)18.6(st)17.2(e)12.8(p)-488.2(re)18.4(ve)18.3(rs)17(e)12.8(-)-487.3(tr)17.8(an)16.1(-)]TJ
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[(sc)18.4(ri)14.7(pt)23.5(io)19(n)-351.1(k)0(i)17.3(t)-348.2(\(Q)21.8(ia)15.9(ge)21(n\))15.1(.)-344.4(E)0(q)17.9(u)0(i)20.4(v)0(a)18.7(l)0(e)15.5(n)0(t)-339(c)13.4(DN)17.6(A)-349.2(a)0(m)15.6(o)0(u)21.2(n)0(t)15.1(s)-349.1(we)23.8(re)-342(us)16.3(e)12.7(d)]TJ
T*
[(to)-262.5(sc)18.4(re)24.7(en)-262.5(e)12.8(x)0(p)15.5(r)0(e)18.4(s)0(s)22.7(i)0(o)19(n)-268.9(of)-256.2(No)16.2(t)12.2(c)0(h)-261.9(s)0(i)20.4(g)0(n)17.5(a)13.1(li)18.2(ng)-254.4(co)16.9(mp)20.1(on)19.1(en)15.7(t)12.2(s)-266.9(us)16.3(in)18.4(g)-263.7(t)0(h)15.1(e)]TJ
0 -1.2266 TD
[(pa)18.1(ir)21.1(s)-443.9(o)0(f)-439.5(p)0(r)23.2(i)0(m)17.9(e)0(r)18.4(s)-443.9(li)18.2(st)17.2(e)12.8(d)-445.6(in)-436.9(S)14.2(u)0(p)16.3(p)0(l)20.4(e)0(m)21.5(e)0(n)15.7(t)0(a)19(r)0(y)-435.1(T)0(a)22.8(b)0(l)17.3(e)-442.5(S1)-434.3(\(S)20(up)16.3(pl)20.4(e-)]TJ
0 -1.2203 TD
[(me)15.2(nt)21.4(ar)18.8(y)-428(D)0(a)24.4(t)0(a)-417.3(a)0(r)18.8(e)-423.5(av)18.7(ai)22.2(la)15.9(bl)23.7(e)-429.9(o)0(n)19.1(l)0(i)18.2(n)0(e)-414.3(a)13.1(t)-430.4(w)0(w)22(w)0(.)20.7(l)0(i)18.2(e)0(b)21(e)0(r)18.4(t)0(p)17.1(u)0(b)19.5(.)0(c)23.1(o)0(m)18.6(/)]TJ
T*
[(sc)18.4(d\))15.5(.)]TJ
1.1128 -1.2203 TD
[(For)-418.9(q-PCR)-421.6(experiments,)-424.1(total)-417.7(RNA)-422.9(\(50100)-168.3(ng,)-415.4(extracted)]TJ
-1.1128 -1.2266 TD
[(from)-248.1(four)-245.6(to)-243.5(six)-247.3(spheres)-248.1(of)-243.5(about)-248.8(the)-244(same)-244.9(size\))-245(were)-248.7(reverse-)]TJ
0 -1.2203 TD
[(transcribed)-294.4(using)-298.5(random)-291.5(hexamer)-294(primers)-289.6(\(Invitrogen\))-300.6(and)]TJ
T*
[(MoMLV)-587.9(reverse)-591.2(transcriptase)-593.2(Superscript)-596.5(III)-581.7(\(Invitrogen\))]TJ
0 -1.2267 TD
[(according)-338.4(to)-332(the)-338.9(manufacturers)-336.2(instructions.)]TJ
1.1128 -1.2203 TD
[(q-PCR)-352.1(were)-349.9(performed)-354.1(using)-355.4(Hes1,)-351.4(Hes5,)-351.4(and)-353.7(Hprt)-350.3(Taq-)]TJ
-1.1128 -1.2203 TD
[(Man)-368.4(Gene)-368.7(expression)-376.7(assays)-367.8(\(Applied)-368.6(Biosystems\))-370.2(and)-366.3(run)]TJ
0 -1.2266 TD
[(on)-398.2(7900HT)-393.1(sequence)-395.2(Detection)-396.8(System)-394.2(\(Fast)-398.2(real)-389.3(time)-399.8(PCR)]TJ
0 -1.2203 TD
[(system;)-652.8(Applied)-652.7(Biosystems\).)-651.3(Three)-651.8(independent)-659.8(experi-)]TJ
T*
[(ments)-502.1(were)-501.6(performed)-505.9(and)-499.1(each)-501.6(sample)-502.7(was)-495.6(screened)-506.9(in)]TJ
0 -1.2266 TD
[(triplicates)-317.1(per)-311.8(individual)-309.7(run.)-311.9(Quantication)-320.4(was)-306(performed)]TJ
0 -1.2203 TD
(using)Tj
/F9 1 Tf
2.858 0 TD
(DD)Tj
/F4 1 Tf
1.3278 0 TD
[(Ct)-443.2(analysis)-433.8(with)-439(specic)-443.9(reference)-440.4(samples)-440.8(as)-437.1(in-)]TJ
-4.1858 -1.2203 TD
[(dicated)-337.5(in)-335.7(gure)-331.8(captions)-340.4(and)-334.7(normalized)-329.6(to)]TJ
/F5 1 Tf
20.227 0 TD
(hprt)Tj
/F4 1 Tf
2.0423 0 TD
(gene.)Tj
/F8 1 Tf
9.9626 0 0 9.9626 59.7543 301.1526 Tm
[(Stati)-10.7(stics)]TJ
/F4 1 Tf
8.9663 0 0 8.9663 69.7323 283.3511 Tm
[(Data)-285.4(are)-291(expressed)-287.7(as)-291.7(mean)]TJ
/F7 1 Tf
12.4245 0 TD
()Tj
/F4 1 Tf
.7208 0 TD
[(SEM.)-287.7(GraphPad)-285.8(Prism)-289(4.0)]TJ
/F5 1 Tf
11.7669 0 TD
(t-)Tj
/F4 1 Tf
-26.0251 -1.2267 TD
[(tests)-427.1(or)-414.5(analyses)-423.9(of)-420.6(variance)-417.6(were)-419.4(used)-422.9(for)-421.3(statistical)-423.9(com-)]TJ
0 -1.2203 TD
[(parisons)-247.8(where)-245.8(appropriate,)-248(with)-243(Dunnetts)-247.5(and)-246.2(Bonferronis)]TJ
T*
[(post)-499.1(hoc)-492.3(tests)-496.7(as)-494(required.)-497(Minimal)-496.7(statistical)-493.5(signicance)]TJ
0 -1.2266 TD
[(was)-337.6(dened)-334.7(at)-335.1(*)]TJ
/F5 1 Tf
7.284 0 TD
(P)Tj
/F7 1 Tf
.7651 0 TD
(<)Tj
/F4 1 Tf
.7208 0 TD
(0.05.)Tj
/F1 1 Tf
9.9626 0 0 9.9626 59.7543 217.5873 Tm
[(Resu)-10.9(lts)]TJ
/F4 1 Tf
8.9663 0 0 8.9663 69.7323 199.729 Tm
[(In)-334.3(the)-338.9(current)-340.9(study,)-335.9(the)-338.9(proliferation)-345.1(of)-332(RSCs)-339.3(was)-337.6(moni-)]TJ
-1.1128 -1.2203 TD
[(tored)-474.7(by)-464.1(evaluating)-471.6(the)-471.6(total)-474.7(numbers)-470.2(of)-464.8(primary)-469.4(or)-471.4(pas-)]TJ
T*
[(saged)-665.7(clonal)-663.3(spheres.)-668.4(The)-663.9(average)-668.6(diameter)-662.2(of)-667.2(clonally)]TJ
0 -1.2266 TD
[(expanded)-366.3(spheres)-361.9(was)-362.9(used)-359.6(as)-361.2(an)-357(estimate)-363.1(of)-363.6(the)-357.8(prolifera-)]TJ
0 -1.2203 TD
[(tion)-294.7(of)-294.1(retinal)-295.6(progenitors)-293.2(\(given)-297(that)-294.7(more)-291.8(than)-291.3(99%)-291.5(of)-294.1(cells)]TJ
T*
[(in)-386.3(the)-389.5(spheres)-393.6(are)-385.8(progenitor)-393.1(cells\))-387.4([23,34].)-384.2(Self-renewal)-397.1(ca-)]TJ
0 -1.2266 TD
[(pacities)-388.3(were)-381.5(assessed)-386.9(by)-381.9(both)-384.1(bulk)-386.8(and)-385.3(single)-382.8(sphere)-385.9(pas-)]TJ
0 -1.2203 TD
[(saging)-743.7(to)-743(address)-742.2(the)-743.5(involvement)-743.1(of)-743(non-cell)-743.4(or)-743.2(cell)]TJ
T*
[(autonomous)-531.8(effects,)-529.5(respectively)-527.2([13,22,23].)-525.2(Moreover,)-527.7(the)]TJ
T*
[(numbers)-426(of)-420.6(new)-415.9(spheres)-425.2(obtained)-421.7(from)-418.8(the)-421.1(dissociation)-422.8(of)]TJ
0 -1.2266 TD
[(individual)-423.5(clonal)-423(spheres)-418.9(enabled)-424.6(the)-421.1(determination)-422.8(of)-420.6(the)]TJ
0 -1.2203 TD
[(mode)-540.7(of)-534.4(division)-534(\(symmetric)-542.7(giving)-532.2(rise)-536.6(to)-534.4(multiple)-542.4(sec-)]TJ
T*
[(ondary)-393.2(clonal)-385.1(spheres)-393.6(or)-382.8(asymmetric)-390(generating)-392.7(one)-385.4(single)]TJ
0 -1.2267 TD
[(clonal)-334.5(secondary)-336.8(sphere\))-342.1(of)-332(the)-332.5(initial)-340(sphere-forming)-336(cell.)]TJ
/F8 1 Tf
9.9626 0 0 9.9626 310.8472 725.0455 Tm
[(Exogenous)-302.8(Dll3)-297.1(Not)-8.2(ch)-291(lig)-7.8(and)-295.8(promote)-9.7(s)-291(prolif)-8.4(eration)]TJ
0 -1.2007 TD
[(of)-333.6(adult)-337.4(RSC)-9.2(s)]TJ
/F4 1 Tf
8.9663 0 0 8.9663 320.8251 692.9573 Tm
[(Notch)-429.5(isoforms)-427.8(\(Notch1)-435.8(to)-426.9(4\))-429.9(represent)-431.3(a)-429.5(class)-428.6(of)-433.2(trans-)]TJ
-1.1128 -1.2203 TD
[(membrane)-470.8(receptors)-480.6(that)-478.1(binds)-470.9(to)-477.5(transmembrane)-476.2(ligands)]TJ
T*
[(\(such)-328.3(as)-323.3(Delta-like)-326.5(1,)-324.9(3,)-324.9(and)-322(4)-328.3(o)0(r)-319.6(Jagged)-327.9(1)-322(and)-328.4(2\))-322.5(present)-329.5(on)]TJ
T*
[(the)-244(surface)-242.8(of)-237.2(adjacent)-244(cells.)-244.4(Receptor)-242.6(ligand-binding)-245.9(initiates)]TJ
0 -1.2266 TD
[(two)-529.7(proteolytic)-537.7(events.)-527.1(The)-531.1(last)-536(one,)-527.5(mediated)-531.7(by)-533.6(the)]TJ
/F9 1 Tf
25.8417 0 TD
(g)Tj
/F4 1 Tf
.4995 0 TD
(-)Tj
-26.3412 -1.2203 TD
[(secretase,)-340.3(releases)-334.9(the)-338.9(NICD,)-341.7(which)-333.7(is)-340(subsequently)-341.9(translo-)]TJ
T*
[(cated)-368.9(to)-376.3(the)-370.5(nucleus)-376.9(where)-372.2(it)-377.1(binds)-369.7(to)-376.3(several)-372.3(partners)-374.1(and)]TJ
0 -1.2266 TD
[(activates)-529.5(the)-528.6(expression)-528.5(of)-528(downstream)-528.7(target)-530.3(Hairy)-526.6(and)]TJ
0 -1.2203 TD
[(enhancer)-382.5(of)-376.3(split)-383.3(\()]TJ
/F5 1 Tf
8.1945 0 TD
(Hes)Tj
/F4 1 Tf
1.5428 0 TD
[(\))-373.5(family)-382.1(genes)-382([35].)-375.9(Several)-382.1(reports)-380.3(in)]TJ
-9.7373 -1.2203 TD
[(both)-403.1(the)-402.1(vertebrate)-398.7(and)-404.2(invertebrate)-405.6(nervous)-402.2(systems)-404.6(have)]TJ
0 -1.2266 TD
[(established)-298.5(a)-303(critical)-300.7(role)-297.8(for)-301.1(Notch)-296.7(signaling)-305.2(in)-297.8(preserving)-300.5(a)]TJ
0 -1.2203 TD
[(pool)-427.7(of)-426.9(undifferentiated)-427.6(precursor)-431.7(cells)-424.9(in)-430.5(developing)-427.2(eyes)]TJ
T*
[([3641].)-374.5(Moreover,)-382.2(we)-374.6(previously)-380(showed)-378.7(that)-376.9(components)]TJ
0 -1.2266 TD
[(of)-401.6(the)-402.1(Notch)-410.6(signaling)-400.1(pathway)-408.6(are)-398.5(critical)-408.2(for)-402.3(the)-408.4(mainte-)]TJ
0 -1.2203 TD
[(nance)-561.9(\(self-renewal\))-565.3(and)-562.3(expansion)-561.1(\(proliferation\))-567.3(of)-566(em-)]TJ
T*
[(bryonic)-334.7(neural)-337.2(and)-328.4(RSCs)-339.3([29,42].)]TJ
1.1128 -1.2203 TD
[(To)-423.1(investigate)-428.2(specic)-431.3(roles)-425.5(of)-426.9(Notch)-429.5(signaling)-431.7(in)-424.2(either)]TJ
-1.1128 -1.2266 TD
[(RSCs)-465.8(or)-458.7(retinal)-466.3(progenitor)-468.9(cells,)-465.7(we)-463.1(rst)-460.7(analyzed)-461.7(the)-465.3(ex-)]TJ
0 -1.2203 TD
[(pression)-248.2(patterns)-247.4(of)-249.8(Notch)-246.2(signaling)-248.3(components)-252.5(\(ie,)-240.8(ligands,)]TJ
T*
[(receptors,)-483.5(and)-480.1(target)-479.7(genes\))-483.6(within)-477.5(the)-484.3(RSC)-477.1(compartment)]TJ
0 -1.2267 TD
[(\(presumptive)-381.7(and)-379(adult)-380.9(ciliary)-381.2(epithelium,)-384.2(pCE)-377.6(and)-379(CE)-376.2(re-)]TJ
0 -1.2203 TD
[(spectively\),)-346.6(in)-348.4(dividing)-347.6(perinatal)-353.3(NR)-345.7(progenitors)-350.1(and)-347.3(in)-348.4(the)]TJ
T*
[(differentiated)-252.2(adult)-248.1(NR.)]TJ
/F5 1 Tf
10.6984 0 TD
[(Notch)-252.2(1)]TJ
/F4 1 Tf
3.1868 0 TD
(,)Tj
/F5 1 Tf
.4932 0 TD
(2)Tj
/F4 1 Tf
.4995 0 TD
(,)Tj
/F5 1 Tf
.4932 0 TD
(3)Tj
/F4 1 Tf
.5058 0 TD
[(,)-243.2(and)]TJ
/F5 1 Tf
2.4154 0 TD
(4)Tj
/F4 1 Tf
.7461 0 TD
[(receptor)-245.3(isoforms)]TJ
-19.0383 -1.2266 TD
[(and)-442.2(the)-446.4(majority)-443.9(of)-445.9(the)-440(Notch)-448.5(ligands,)-445.2(including)]TJ
/F5 1 Tf
22.8257 0 TD
[(Jagged)-447.9(1)]TJ
/F4 1 Tf
3.5914 0 TD
(,)Tj
/F5 1 Tf
-26.4171 -1.2203 TD
[(Jagged)-492.2(2)]TJ
/F4 1 Tf
3.6357 0 TD
(,)Tj
/F5 1 Tf
.7398 0 TD
(Delta-like)Tj
/F4 1 Tf
4.3691 0 TD
(\()Tj
/F5 1 Tf
.3225 0 TD
(Dll)Tj
/F4 1 Tf
1.3088 0 TD
(\))Tj
/F5 1 Tf
.3225 0 TD
(1)Tj
/F4 1 Tf
.4995 0 TD
[(,)-496.1(and)]TJ
/F5 1 Tf
2.9149 0 TD
(Dll4)Tj
/F4 1 Tf
1.8084 0 TD
[(,)-489.8(were)-495.3(ubiquitously)-494(ex-)]TJ
-15.9211 -1.2203 TD
[(pressed)-418.5(in)-417.9(both)-422(developing)-420.9(\(PND2\))-420.5(and)-416.9(adult)-418.9(CE)-420.5(and)-416.9(NR)]TJ
0 -1.2266 TD
[(\(Fig.)-433.1(1B\).)-429.5(By)-431.1(contrast,)]TJ
/F5 1 Tf
10.2621 0 TD
(Dll3)Tj
/F4 1 Tf
2.2446 0 TD
[(is)-428.5(expressed)-433.2(only)-431.4(in)-436.9(PND2)-432.2(NR)]TJ
-12.5067 -1.2203 TD
[(progenitor)-247.6(cells)-241.5(and)-239.8(no)-246.4(longer)-242.3(in)-240.9(adult)-248.1(NR,)-241.1(nor)-240.8(in)-247.2(pCE)-238.5(or)-243.7(CE.)]TJ
T*
[(Regarding)-269.7(the)-269.3(Notch)-271.5(target)-277.4(genes,)-271.1(only)]TJ
/F5 1 Tf
17.4828 0 TD
(Hes5)Tj
/F4 1 Tf
2.3079 0 TD
[(was)-274.3(specically)]TJ
-19.7907 -1.2203 TD
[(expressed)-294.1(in)-291.5(the)-294.6(PND2)-299.4(NR)-288.7(and)-296.8(not)-291.2(in)-291.5(the)-294.6(other)-298.1(tested)-295.9(tissue)]TJ
0 -1.2266 TD
[(samples)-459.7(\(Fig.)-458.4(1B\).)-461.2(This)-460.2(correlation)-465.5(between)]TJ
/F5 1 Tf
20.2143 0 TD
(Hes5)Tj
/F4 1 Tf
2.5039 0 TD
(and)Tj
/F5 1 Tf
2.1371 0 TD
(Dll3)Tj
/F4 1 Tf
-24.8554 -1.2203 TD
[(expression)-414.7(patterns)-418.1(suggests)-412.8(a)-410.5(possible)-410.2(communication)-419.1(be-)]TJ
T*
[(tween)-270.8(neighboring)-274.1(PND2)-267.8(NR)-269.8(progenitors)-274.2(as)-272.7(Dll3,)-270.1(binding)-272.3(to)]TJ
0 -1.2266 TD
[(Notch)-530.7(receptor-expressing)-542(progenitors,)-536.4(which)-529.7(would)-530.8(acti-)]TJ
0 -1.2203 TD
[(vate)-481.2(downstream)]TJ
/F5 1 Tf
8.3273 0 TD
(Hes5)Tj
/F4 1 Tf
2.5228 0 TD
[(expression.)-480.8(This)-479.1(result)-481.5(is)-479.1(in)-481.1(accor-)]TJ
-10.8501 -1.2203 TD
[(dance)-295.9(with)-299.9(a)-303(previous)-302.5(study)-295.1(showing)-305(that)]TJ
/F5 1 Tf
19.0003 0 TD
(Hes5)Tj
/F4 1 Tf
2.3395 0 TD
[(is)-295.7(expressed)]TJ
-21.3398 -1.2266 TD
[(in)-266.2(dividing)-271.7(retinal)-270.3(precursors)-275(located)-267.7(in)-272.5(the)-269.3(germinal)-265.5(zone)-271.1(in)]TJ
0 -1.2203 TD
[(developing)-243.8(mouse)-243.2(retina,)-244.4(before)-248.4(being)-243.3(gradually)-241.3(restricted)-249.5(to)]TJ
T*
[(differentiating)-412.4(Mu)]TJ
7.7266 .0063 TD
()Tj
.4679 -.0063 TD
[(ller)-412.3(glial)-406.5(cells)-412.2(later)-408.8(in)-411.6(the)-408.4(early)-412.7(postnatal)]TJ
-8.1945 -1.2203 TD
[(\(PND7\))-338.3(period)-333.7([43].)]TJ
1.1128 -1.2267 TD
[(We)-504.8(n)-9.7(e)-12.5(x)-8.5(t)-500(e)-12.5(x)-8.5(a)-12.2(m)0(i)-20.1(n)-9.7(ed)-508.7(th)-16.5(e)-499.4(f)-13.1(u)-7.7(n)-9.7(c)-11.9(t)-13.1(i)-9.9(o)-9.1(n)-9.7(a)-12.2(l)-496.7(e)-12.5(ff)-19.9(e)-12.5(c)-11.9(ts)-507.6(o)-9.1(f)-500(ex)-21(o)-9.1(g)-10.7(e)-12.5(n)-9.7(o)-9.1(u)-7.7(s)]TJ
-1.1128 -1.2203 TD
.0111 Tc
[(Dll-induce)11.1(d)-636.7(N)-1.5(otc)11.1(h)-636.5(act)11.1(i)-5.5(vat)11.1(i)-5.5(o)-4.3(n)-624.6(o)-4.3(n)-630.9(de)11.1(v)-8.5(e)-1.4(lopin)-4.9(g)-625.6(PN)11.1(D)-9.2(2)-627(NR)]TJ
T*
0 Tc
[(p)-7.7(r)-13.3(o)-9.1(g)-10.7(e)-12.5(n)-9.7(i)-9.9(t)0(o)-22.2(r)-7(s)-570.4(a)-12.2(n)-9.7(d)-565.7(P)-14(N)0(D)-20.4(2)-568.6(R)-10.6(S)-11.1(Cs)-576.9(r)-13.3(e)0(s)-20.2(p)-7.7(e)-12.5(c)-11.9(ti)-16.7(v)-13.4(e)-12.5(l)-9.9(y)-10.7(,)-565.7(a)-12.2(n)-9.7(d)-565.7(o)-9.1(n)-572.4(a)-12.2(d)-9.3(u)-7.7(l)-9.9(t)]TJ
0 -1.2267 TD
.0105 Tc
[(RSC)10.5(s)-9.9(.)-378.1(N)10.5(e)-8.3(i).6(ther)-375.9(the)-381.4(a)10.5(d)-4.7(d)-5.2(it)10.5(i)-6.1(o)1.4(n)-384.9(o)1.4(f)-375.7(D)-3.5(ll1)-381(nor)-375.9(D)-3.5(ll3)-374.7(pro)-4.9(d)1.2(uced)-378.2(sig-)]TJ
0 -1.2203 TD
0 Tc
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T*
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