The Sidhu lab studies the relationships between protein structure and function, using phage display in conjunction with high-throughput screening and sequencing. By studying the basic principles behind phage display technology, we have greatly improved library diversities and the scaffolds used for protein display. We have applied these advances to the development of synthetic antibody libraries, which we and others are currently using for the development of therapeutic antibodies for unmet medical needs. We have also extended our phage display, high throughput methodology to the generation of synthetic peptides that can be used to modulate enzyme function. In addition, we have been studying peptide-binding modules to gain insight into the biological function of intracellular scaffolding proteins.

Our research is concentrated on the following two areas:

1. High throughput, phage display-based generation of synthetic antibodies

2. Protein-protein interactions: Large-scale analysis of peptide recognition modules & high-throughput generation of peptide-based binders.